Research interests

• Atmospheric pressure ionization mass spectrometry: Mass spectrometry using ionization at atmospheric pressure to efficiently produce molecular ions. (Holds the patent for this method.)
• Field desorption ionization and collision induced decomposition mass spectrometer for biological molecules.
• Molecular secondary ion mass spectrometry: molecular ion production and mass spectrometry by ion bombardment ionization for non-volatile bio compounds. (1983 IR100)
• Combined system of liquid chromatograph and mass spectrometer. (1994 R&D 100)
• Fluorescent DNA sequencer
• Capillary array DNA sequencer. (Holds many patents on DNA detection systems)
• Technologies for analyzing gene expressions as well as DNA diagnostics.
• Technologies and instruments for single cell and tissue analysis.

Construction of an information system just like Google Earth for the human body.

Major research activity

• 1982-present Development of DNA analysis technology
  Development of the DNA sequencer, gene expression profile analysis method, gene mutation measurement technology, capillary array DNA analysis equipment, SNPs-oriented DNA mutation analysis methods, probe array method using beads, etc.
• 1973-1989 Development of ionization technology for biological samples
  Development of Atmospheric Pressure Ionization Mass Spectrometry, Field Ionization, Molecular-SIMS, LC/MS (Liquid Chromatography/Mass Spectrometer combined device), etc.

Major publications

1. T.Yoda, M.Hosokawa, K.Takahashi, C.Sakanashi, H.Takeyama and H.Kambara, “Site-specific gene expression analysis using an automated tissue micro-dissection punching system,” Scientific Reports 7: 4325(2017).
2. T.Kajiyama, A.Fujii, K.Arikawa, T.Habu, N.Mochizuki, A.Nagatani and H.Kambara, ”Position-Specific Gene Expression Analysis Using a Microgram Dissection Method Combined with On-Bead cDNA Library Construction,” Plant & Cell Physiology 56 (2015): pp. 1320-1328.
3. H.Matsunaga, M.Goto, K.Arikawa, M.Shirai, H.Tsunoda, H.Huang and H.Kambara, “A highly sensitive and accurate gene expression analysis by sequencing (“bead-seq”) for a single cell,” Anal. Biochem 471, pp. 9-16 (2015).
4. K.Taniguchi, T.Kajiyama and H.Kambara, “Quantitative analysis of gene expression in a single-cell by qPCR,” Nature Method 6, pp. 503-506(2009).
5. G.Zhou, M.Kamahori, K.Okano, K.Harada and H.Kambara, ”Miniaturized pyrosequencer for DNA analysis with capillaries to deliver deoxynucleotides,” Electrophoresis 22(2001): pp. 3497-3504.
6. G.Zhou, M.Kamahori, K.Okano, G.Chuan, K.Harada, and H.Kambara,”Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER),” Nucleic Acid Res. 29(2001): No. 19 e93.
7. Y.Kohara, H.Noda, K.Okano and H.Kambara, ”DNA hybridization using “Bead-Array”: Probe-attached beads arrayed in a capillary in a predetermined order” Nucleic Acid Res. suppl 1 (2001): pp. 83-84.
8. T. Anazawa, S.Takahashi and H.Kambara, “A capillary array gel electrophoresis system using multiple laser focusing for DNA sequencing,” Anal. Chem. 68 (1996): pp. 3699-2704.
9. H.Kambara and S.Takahashi, “Multiple-sheath flow capillary array DNA analyser”, Nature 361, pp. 565-566 (1993).
10. U.S. Patent US6224733 B1, Japanese Patent P2974495（Priority claim date: 24th April 1992: DNA detector and DNA detection method, by S. Takahashi and H. Kambara.
11. M.Sakairi and H.Kambara, “Atmospheric Pressure Spray Ionization for Liquid Chromatography/Mass spectrometry,” Anal. Chem. 61 (1989): pp. 1159-1164.
12. H.Kambara, T.Nishikawa, Y.Katayama and T.Yamaguchi, ”Optimization of parameters in a SNA sequenator using fluorescence detection” Bio/Technology 6, 816-821(1988).
13. Japanese Patent P2013548 (Priority claim date: 1st February 1984) JP1994043135: Device for determining base sequence of nucleic acids, by H. Kambara, O. Okada, et al. (9th February 1994 Patent Application Notification No. 特公平6-10665; 1st February 1984 Patent Application No.特願昭59-15226)
14. M.Sakairi and H.Kambara, “Characteristics of a Liquid Chromatograph/Atmospheric Pressure Ionization Mass Spectrometer”, Anal Chem 60 (1988): pp. 774-780.
15. K.Harada, M.Suzuki and H.Kambara, “Diethanolamine Assisted Secondary Ion Mass Spectrometry of Naturally Occurring Complex Oligosaccharides,” Org. Mass Spectrom. 17 (1982): pp. 386-391.
16. H.Kambara and S.Hishida, “Secondary Ion Mass Spectra of Nonvolatile Bioorganic Compounds,” Anal Chem. 53 (1981): pp. 2340-2344.
17. H.Kambara and I.Kanomata, “Determination of Impurities in Gases by Atmospheric pressure Ionization Mass Spectrometry,” Anal. Chem. 49 (1977): pp. 276-275.